The impact of size and position of reference electrode on the localization of biphasic electrotactile stimulation on the fingertips

Development of haptic interfaces to enrich augmented and virtual reality with the sense of touch is the next frontier for technological advancement of these systems. Among available technologies, electrotactile stimulation enables design of high-density interfaces that can provide natural-like sensation of touch in interaction with virtual objects. The present study investigates the human perception of electrotactile sensations on fingertips, focusing on the sensation localization in function of the size and position of reference electrode. Ten healthy subjects participated in the study, with the task to mark the sensations elicited by stimulating the index fingertip using an 8-pad electrode. The test systematically explored several configurations of the active (position) and reference (position and size) electrode pads. The results indicated that there was a spreading of perceived sensations across the fingertip, but that they were mostly localized below the active pad. The position and size of the reference electrode were shown to affect the location of the perceived sensations, which can potentially be exploited as an additional parameter to modulate the feedback. The present study demonstrates that the fingertip is a promising target for the delivery of high-resolution feedback.The work in this study was performed within the TACTILITY project, which has received funding by European Union’s Horizon 2020 framework programme for research and innovation H2020-ICT-2018-2020/H2020-ICT 2018-3 under grant agreement no. 85671

A compact system for simultaneous stimulation and recording for closed-loop myoelectric control

Background.Despite important advancements in control and mechatronics of myoelectric prostheses, the communication between the user and his/her bionic limb is still unidirectional, as these systems do not provide somatosensory feedback. Electrotactile stimulation is an attractive technology to close the control loop since it allows flexible modulation of multiple parameters and compact interface design via multi-pad electrodes. However, the stimulation interferes with the recording of myoelectric signals and this can be detrimental to control.The work in this study was supported by the project ROBIN (8022-00243A and 8022-00226B) funded by the Independent Research Fund Denmark

High-throughput roll-to-roll production of polymer biochips for multiplexed DNA detection in point-of-care diagnostics

Roll-to-roll UV nanoimprint lithography has superior advantages for high-throughput manufacturing of micro- or nano-structures on flexible polymer foils with various geometries and configurations. Our pilot line provides large-scale structure imprinting for cost-effective polymer biochips (4500 biochips/hour), enabling rapid and multiplexed detections. A complete high-volume process chain of the technology for producing structures like μ-sized, triangular optical out-couplers or capillary channels (width: from 1 μm to 2 mm, height: from 200 nm up to 100 μm) to obtain biochips (width: 25 mm, length: 75 mm, height: 100 μm to 1.5 mm) was described. The imprinting process was performed with custom-developed resins on polymer foils with resin thicknesses ranging between 125–190 μm. The produced chips were tested in a commercial point-of-care diagnostic system for multiplexed DNA analysis of methicillin resistant Staphylococcus aureus (e.g., mecA, mecC gene detections). Specific target DNA capturing was based on hybridisation between surface bound DNA probes and biotinylated targets from the sample. The immobilised biotinylated targets subsequently bind streptavidin–horseradish peroxidase conjugates, which in turn generate light upon incubation with a chemiluminescent substrate. To enhance the light out-coupling thus to improve the system performance, optical structures were integrated into the design. The limits-of-detection of mecA (25 bp) for chips with and without structures were calculated as 0.06 and 0.07 μM, respectively. Further, foil-based chips with fluidic channels were DNA functionalised in our roll-to-roll micro-array spotter following the imprinting. This straightforward approach of sequential imprinting and multiplexed DNA functionalisation on a single foil was also realised for the first time. The corresponding foil-based chips were able to detect mecA gene DNA sequences down to a 0.25 μM concentration.This research was supported by R2R BIOFLUIDICS project (http://www.r2r-biofluidics.eu/) under Horizon 2020 European Union (EU) Research and Innovation Programme with grant agreement no 646260. The research was also partially supported by NextGenMicrofluidics project (https:// www. nextgenmicrofluidics.eu/) under HORIZON2020 with grant agreement no 862092. The authors cordially thank Gerburg Schider & Gerhard Mohr, Markus Postl, Paul Patter and Alexander Wheeldon (JOANNEUM RESEARCH – Materials, Weiz, Austria) for revising the manuscript, preparing all the chip and R2R pilot line illustrations, taking the photographs and providing technical support, respectively. The authors are also grateful to Christian Wolf and Johannes Götz (JOANNEUM RESEARCH – Materials, Weiz, Austria) for their supports in the fluidic design and R2R UV-NIL structuring, respectively. We further kindly thank Alba Simon Munoz and Robert Fay (SCIENION AG, Berlin, Germany) for providing the illustration of the R2R micro-spotting line. PT specially thanks Ege Ozgun (NANOTAM, Bilkent University, Ankara, Turkey) for critically reading the manuscript

Artificial Skin and Electrotactile Stimulation for Advanced Tactile Feedback in Myoelectric Prostheses

none5siModern prosthetic arms and hands are sophisticated robotic devices that can provide some of the motor functions lost due to an amputation. However, none of the commonly used commercial systems restores somatosensory feedback to its user. In principle, the latter can be achieved by recording data from prosthesis sensors and conveying this information by stimulating the sensory structures of the amputee using invasive and noninvasive interfaces. Many such systems have been presented in the literature with promising results; however, they all rely on using several stimulation points to transmit information and hence suffer from a limited information bandwidth. In this chapter, we propose a novel concept of a high-bandwidth feedback interface that relies on advanced sensing and stimulation to convey a large amount of information to the prosthesis user. The interface comprises an artificial skin covering the prosthesis with a dense network of tactile sensors (taxels) and a compact stimulation device delivering electrical current pulses through a matrix electrode with many conductive pads. The state-of-the-art in the two technologies that are required for the implementation of the proposed concept are reviewed. This includes biomimetic e-skins that are suitable for the application in a wearable scenario, stimulation systems integrating a demultiplexing circuit to distribute electrical pulses, and flexible electrodes that can be produced with arbitrary shape, size, and distribution of conductive pads. Finally, the challenges in selecting feedback variables (raw signals versus high-level features) and mapping of these variables into stimulation parameters are addressed.mixedLucia Seminara, Matija Strbac, Youssef Amin, Maurizio Valle, Strahinja DosenSeminara, Lucia; Strbac, Matija; Amin, Youssef; Valle, Maurizio; Dosen, Strahinj